DEVELOPMENT OF THE BIOCATALYTIC SYNTHESIS OF MARESIN
As it has been told and due to the COVID19 pandemic, Maresin (a naturally occurring antiinflammatory polyunsaturated fatty acid synthesized by the macrophague cells of the (human) immunity system) has attracted again the interest of researchers and pharma industry because of its role and great potential in the treatment of severe acute respiratory syndromes such as COVID. Maresin has also proven activity in the treatment of ELA and other sclerosis illnesses, but its extreme difficult chemical synthesis has been blocking its development as an API.
Biochemize SL is working in the development of the biocatalytic synthesis of Maresin (see our previous notes), and the second hypothetic enzyme of the pathway -that has been designed through computational modelling and quantic mechanics, at the Department of Chemistry of the Autonomous University of Barcelona- has finally been succesfully expressed, together with the Biotechnology and Biomedicine Institute at the Autonomous University of Barcelona.
Now we are starting to check its activity and combine with the activity of the first enzyme of the pathway, and facing the last challenge to be able to produce low cost Maresin.
Date: May 2021
Biochemize SL has received the Innovative SME label granted by the Spanish MICINN
Due to the research activity developed and the results obtained, Biochemize SL has been awarded with the Innovative SME label, that grants the Spanish MICINN. This is an extra reason to keep on working hard. #research #publishing #innovation
Date: May 2021
NEW RESEARCH PROJECT TO BE STARTED, FUNDED BY EU (PROGRAM BBI)
OLEAF4VALUE: An Innovative Upcycling Project. Natac is coordinating the OLEAF4VALUE project, a research and innovation action (RIA) funded by the European Union (H2020 -JTI-BBI) focused on tackling the problem generated by olive biomass through the development of a complete olive leaf upcycling system.
Olive LEAF multi-product cascade based biorefinery: from an under-used biomass in the primary sector to tailor-made solutions with high added VALUE for international market applications.
The OLEAF4VALUE consortium is composed of sixteen partners: six Research Technology Organisations (RTOs), seven Small and Medium Enterprises (SMEs), and three Large Enterprise (Les), from nine different countries throughout Europe (Germany, Italy, Netherlands, Norway, Portugal, Slovenia, Spain, Switzerland and UK).
OLEAF4VALUE is a three-year project that will develop a complete valorisation system for the olive leaf. Globally, 4.5 million tons of olive leaves are produced every year by the olive oil industry, a key industry in southern Europe and along the Mediterranean basin. This biomass represents a challengefor both the farmers and the whole olive oil industry, who need to remove it from the fields and the olive oil mills. Olive leaves may generatecurrently an environmental and economic problem when not collected. OLEAF4VALUE will assemble a competitive consortium of highly experienced partners* devoted to the complete valorisation of this underexploited raw material.
NATAC BIOTECH SL (NATAC)
OLEICOLA EL TEJAR NUESTRA SENORA DEARACELI S COOP AND (OET)
INGECOR AGROFORESTAL SL (IGC)
INNORENEW COE CENTER ODLICNOSTI ZA RAZISKAVE IN INOVACIJE NA PODROCJU OBNOVLJIVIH MATERIALOV IN ZDRAVEGA BIVANJSKEGA OKOLJA (INNO)
FUNDACION CENER (CENER)
INSTITUTO POLITECNICO DE BRAGANCA (IPB)
BIOCHEMIZE SL (BCZ)
UNIVERSITA DEGLI STUDI DI FIRENZE (UNIFI)
NIZO FOOD RESEARCH BV (NIZO)
MARTIN-LUTHER-UNIVERSITAET HALLE-WITTENBERG (MLU)
MIBELLE AG (MBL)
EURIZON SL (Innovarum)
ZERO EMISSIONS ENGINEERING BV (Zero-E)
NNFCC LIMITED (NNFCC)
MOWI FEED AS (MOWI)
Date: April 2021
Biocatalytic synthesis of maresin. New modelling approach based on Leukotriene A4 Hydrolase.
Biochemize continues with the development of the biocatalytic synthesis of maresin. Once the lipoxygenase step is running very well with different versions of recombinant microbial (E coli) expressed 12Lox and 15Lox (which is supported with significant and new bibliography); the work is being finished wiht the second step hydrolisis reaction. Modelling studies focus on the reaction mechanism corresponding to the hydrolisis of Leukotriene A4 methyl ester (LTA4) to Leukoriene B4 (LTB4) mediated by the enzyme Leukotriene A4 Hydrolase (LTA4H). The image attached shows the placement of the substrate LTA4 inside the catalytic centre of the enzyme. All the aminoacidic residues involved on the reaction have been identified, and also all the residues related to the action of the atom of Zn. The epoxydic ring can be appreciated before its breaking, on the upper side of the picture. #research #maresin #leukotriene A4 hydrolase #biocatalysis